Analysis Note

ControlU2Os cell lysate

Application

Immunocytochemistry Analysis: A representative lot detected PLK-4 in U20S cell lysates transfected with control and Cep152 siRNA, but not U2OS cell lysates transfected with PLK4 siRNA. (Cizmecioglu, O. et al. (2010). J Cell Biol. 191(4):731-739).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PLK-4 in HeLa cell lysates cotransfected with Flag-tagged Gcp6 and Myc-tagged Plk-4 (Bahtz, R. et al. (2012). J Cell Sci. 125 (Pt 2): 486-496).

Research CategoryApoptosis & Cancer

Detect Polo Like Kinase (PLK) using this mouse monoclonal antibody, Anti-PLK-4 Antibody, clone 6H5 validated for use in western blotting, ICC & IP.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Serine/threonine-protein kinase PLK4 (PLK-4) is a member of the protein kinase superfamily and plays an essential role in centriole duplication. PLK-4 triggers procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins. It may be involved in tumorigenesis since centrosome aberrations are frequently observed in tumors. PLK-4 may also be involved in trophoblast differentiation by phosphorylating HAND1, leading to the disruption between HAND1 and MDFIC and then activating HAND1.

Immunogen

Linear peptide corresponding to Human PLK-4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in U2Os cell lysate.

Western Blotting Analysis: 4.0 µg/mL of this antibody detected PLK-4 in 10 µg of U2Os cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

109 kDa observed